NucleoSpin 96 DNA Stool enables high-throughput isolation of both microbial and host genomic DNA from fresh and frozen stool samples in a 96-well format. The kit employs a special lysis buffer in combination with mechanical sample disruption using MN Bead Tubes Type A (ceramic beads; included in the kit) to achieve efficient sample homogenization without enzymatic reactions such as protease digestion. Automation of the kit protocol is possible on common liquid handling platforms.