Short-read NGS technologies have dramatically accelerated the pace of biological discovery in recent years, but for applications such as genome assembly, analysis of repetitive regions, and genome phasing, longer-read approaches are often a better fit. The NucleoBond HMW DNA kit was specifically developed for long-read sequencing applications, and employs gravity flow filtration in combination with NucleoBond anion-exchange technology to enable efficient recovery of high molecular weight genomic DNA of the highest purity and integrity from diverse samples.