The NucleoSpin RNA Stool kit is designed for efficient isolation of RNA, including small RNA species, from fresh and frozen stool samples. The kit combines NucleoZol with Lysis Buffer RST1 for chemical disruption of stool and associated microbes. The mechanical lysis is performed using MN Bead Tubes Type A (containing ceramic beads) in combination with a mechanical disruption device. No enzymatic reactions, such as protease digestion, are required to homogenize the sample material.