NucleoSpin 8 RNA is designed for automated isolation and purification of total RNA from animal or human cell cultures and tissue. With the NucleoSpin 8 RNA method, cells are lysed in the presence of chaotropic salts. The lysis buffer immediately inactivates RNases, which are present in virtually any biological material, and creates appropriate conditions for RNA to bind to the silica membrane. Genomic DNA is removed by performing on-column DNA digestion using the rDNase provided in the kit.