NucleoSpin RNA XS allows the isolation of highly concentrated, highly pure total RNA from very dilute or low-volume tissue and cell samples such as biopsy material or single cells. The innovative funnel-shaped thrust ring allows standard miniprep spin columns to hold a silica membrane of very small diameter. The small surface of the NucleoSpin RNA XS membrane allows efficient elution in only 5–20 µl, resulting in highly concentrated RNA. Purified RNA is ready for all typical downstream applications.