NucleoZOL is a highly efficient RNA-isolation reagent for extracting both small and large RNAs from a variety of sample types, such as cultured cells, animal and plant tissue, yeast, bacteria, and viruses. A simple, one-phase extraction with NucleoZOL eliminates the need for an inconvenient two-phase separation using chloroform, which, in turn, minimizes the risk of sample loss and phenol carryover. DNA, protein, and other contaminants are removed by precipitation.