The CellAmp Direct Probe RT-qPCR Kit is designed for performing a simple two-step real-time RT-qPCR, without RNA extraction, directly from various animal cells (e.g., adherent cells, suspension cells, primary cells, various stem cells, and iPS cells). The kit makes it possible to prepare template samples from cultured cells and complete reverse transcription reactions and gene expression analysis in as little as 1.5 hours.