The CellAmp Direct TB Green RT-qPCR Kit is designed for performing a simple two-step real-time RT-PCR, without RNA extraction, directly from various animal cells (e.g., adherent cells, floating cells, primary cells, various stem cells, and iPS cells). The kit makes it possible to prepare template samples from cultured cells and complete the analysis of reverse transcription reactions and gene expression in as little as 1.5 hr.