Prelude One-Step PreAmp Master Mix uses an optimized reverse transcriptase, polymerase, and buffer to maximize RT-PCR efficiency for all targets in a preamplification reaction. This enables the highest level of unbiased preamplification for >100 targets, from as little as 100 pg mRNA or 10 ng total RNA. This 2X premix works with intact RNA or degraded RNA extracted from FFPE samples. Amplified products can be used for downstream real-time PCR (qPCR), genotyping, or target-enrichment analysis.