Premix Ex Taq DNA Polymerase (Perfect Real Time) is a 2X RT-PCR kit specifically designed for fast and sensitive real-time PCR via either intercalating green dye Real-Time PCR (qPCR) or probe-based qPCR assays. TB Green dye and probes for probe/5' nuclease-based assays are not included in this RT-PCR kit. The Premix Ex Taq DNA Polymerase (Perfect Real Time) kit consists of our high-fidelity and high-performance Takara Ex Taq Hot Start polymerase and a real-time buffer that ensures superior specificity and increased amplification efficiency during RT-PCR (qPCR). Antibody-mediated hot-start technology prevents nonspecific amplification due to mispriming and/or the formation of primer dimers during room-temperature reaction assembly. Once the Taq antibody-polymerase complex is denatured during the first cycling step, the Premix Ex Taq polymerase can initiate DNA synthesis.