Probe qPCR Mix, with UNG is designed for probe-based qPCR. A combination of a hot-start PCR enzyme and an optimized buffer results in excellent suppression of nonspecific amplification, high amplification efficiency, and high detection sensitivity in real-time PCR analyses. This product is also suitable for high-speed PCR. It allows accurate target quantification and detection over a broad dynamic range and makes it possible to perform highly reproducible and reliable real-time PCR analyses.