TB Green Premix Ex Taq (Tli RNase H Plus), an intercalating dye-based qPCR master mix for real-time RT-PCR (qPCR), is recommended for most real-time PCR applications including gene expression analyses, genotyping, and high-throughput studies. TB Green Premix Ex Taq (Tli RNase H Plus) includes TB Green, a qPCR reagent for intercalator-based RT-PCR (qPCR). In addition to being ideal for most standard real-time PCR reactions, this kit is recommended when a higher yield of PCR product is needed or when amplifying longer products (up to 570 bp). This TB Green qPCR premix is supplied at a 2X concentration and allows easy reaction assembly; by simply adding primers, template, and sterile distilled water, intercalator-based qPCR can be performed.