When synthesizing cDNA for real-time PCR, the PrimeScript RT Reagent Kit (Perfect Real Time) allows maximal flexibility during reaction assembly. This reverse transcription reagent kit facilitates cDNA synthesis prior to analysis by real-time PCR. Designed for two-step real-time RT-PCR (also called qRT-PCR or RT-qPCR), the PrimeScript RT Reagent Kit (Perfect Real Time) contains all of the components needed for reverse transcription, including PrimeScript RTase, RNase inhibitor, random 6-mers, oligo dT primer, dNTPs, and reaction buffer. Because the kit's primers and RTase are provided as separate components, there is greater flexibility in reaction assembly.